Biochemical properties of attachment region binding protein ARBP

FEBS Letters

Buhrmester, Hartmut¹  Strätling, Wolf H.¹  Rosorius, Olaf¹  von Kries, Jens P.¹ 

[1] Institut für Physiologische Chemie, Universitäts-Krankenhaus Eppendorf, Martinistr. 52, D-20246 Hamburg, Germany

关键词: Chromatin organization;    ARBP;    DNA binding domain;    Sedimentation coefficient;    Stokes radius;   

DOI  :  10.1016/0014-5793(94)80497-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

ARBP (attachment region binding protein) is an abundant nuclear protein that specifically binds to matrix/scaffold attachment regions (MARs/SARs). Here we show by gel filtration and gradient sedimentation that ARBP has an elongated shape. The sedimentation coefficient was determined as only 2.1 S. Furthermore, limited proteolysis of ARBP in situ (in isolated nuclei) with several proteases generated limiting resistant peptides from 14.5 to 18 kDa, that retained the ability to bind MARs specifically. This indicates that these peptides encompass the DNA binding domain of ARBP.

【 授权许可】

Unknown