Three‐dimensional crystal structure of recombinant erabutoxin a at 2.0 Å resolution

FEBS Letters

Ménez, Renée¹  Arnoux, Bernadette²  Drevet, Pascal¹  Boulain, Jean-Claude¹  Ménez, André¹  Ducruix, Arnaud² 

[1] Département d'Ingénierie et d'Etudes des Protéines, DSV, CEA, CE Saclay, 91191, Gif-sur-Yvette Cedex, France;Laboratoire de Biologie Structurale, CNRS, 91198, Gif-sur-Yvette Cedex, France

关键词: Recombinant snake toxin;    Crystal structure;    Ea;    Erabutoxin a;    Ear;    recombinant erabutoxin;    Eb;    erabutoxin b;    AcChoR;    acetylcholine receptor;   

DOI  :  10.1016/0014-5793(94)80574-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recombinant erabutoxin a (Ea_r) has been crystallized by vapour diffusion in hanging drops. The crystals belong to space group P2₁2₁2₁ with cell dimensions a = 55.8 Å, b = 53.4 Å, c = 40.8 Å. Diffraction data have been recorded on a FAST detector up to 2.0 Å. The atomic crystal structure of Ea_r has been determined by initial refinement of the structure of the isotoxin erabutoxin b (Eb) the crystals of which were grown under identical conditions. The R-factor was 23% at 2.0 Å resolution. The secondary and tertiary structures of Ea_r are shown to be identical with that of wild-type Eb, within the experimental error.

【 授权许可】

Unknown