A novel prenyltransferase, farnesylgeranyl diphosphate synthase, from the haloalkaliphilic archaeon, Natronobacterium pharaonis

FEBS Letters

Tachibana, Akira¹ 

[1] Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, Osaka 558, Japan

关键词: Prenyltransferase;    Farnesylgeranyl diphosphate synthase;    Geranylgeranyl diphosphate synthase;    Archaeal lipid;    Haloalkaliphile;    Natronobacterium pharaonis;    DMAPP;    dimethylallyl diphosphate;    FGPP;    farnesylgeranyl diphosphate;    FPP;    farnesyl diphosphate;    GGPP;    geranylgeranyl diphosphate;    GPP;    geranyl diphosphate;    IPP;    isopentenyl diphosphate;   

DOI  :  10.1016/0014-5793(94)80475-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel prenyltransferase, farnesylgeranyl diphosphate (FGPP) synthase (EC 2.5.1.X), which synthesizes C₂₅-prenyl diphosphate, was found in the haloalkaliphilic archaeon Natronobacterium pharaonis. It was separated from geranylgeranyl diphosphate (GGPP) synthase (EC 2.5.1.29), which synthesizes C₂₀-prenyl diphosphate, a major prenyltransferase in this organism. The highest activity of FGPP synthase was observed when GGPP was used as the allylic substrate. FGPP synthase may synthesize a precursor for the C₂₅ moiety of C₂₀,C₂₅ diether lipids using a longer allylic diphosphate, such as GGPP synthesized by GGPP synthase, rather than dimethylallyl diphosphate, which is the product of isopentenyl diphosphate isomerase.

【 授权许可】

Unknown