Yeast cells with a specific cellular make‐up and an environment that removes acetaldehyde are prone to sustained glycolytic oscillations

FEBS Letters

Westerhoff, Hans V.¹  Teusink, Bas¹  Richard, Peter¹  van Dam, Karel¹  Bakker, Barbara M.¹  Diderich, Jasper A.¹ 

[1] E.C. Slater Institute, BioCentrum, University of Amsterdam, Plantage Muidergracht 12, NL-1018 TV Amsterdam, The Netherlands

关键词: S. cerevisiae;    Signalling;    Yeast glycolysis;    Flux control;    Regulation;    Limit cycle;    Growth phase independence;   

DOI  :  10.1016/0014-5793(94)80461-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glycolytic oscillations can be induced by adding glucose to starved Saccharomyces cerevisiae cells and, after a steady state has been established, cyanide. Transient oscillations or limit-cycle oscillations can be induced depending on the growth phase in which the cells are harvested. To find what causes these differences in the dynamic behaviour, we analyzed glycolytic enzyme activities at different growth phases. The hexokinase activity increased by a factor of three after growth substrate transition from glucose to ethanol; the other measured activities remained constant. Cyanide was found not only to block respiration, but also to trap acetaldehyde. Both cyanide actions appear necessary for the occurrence of sustained glycolytic oscillations.

【 授权许可】

Unknown