Both interleukin‐8 receptors independently mediate chemotaxis

FEBS Letters

Clark-Lewis, Ian³  Moser, Bernhard²  Seitz, Michael¹  Baggiolini, Marco²  Loetscher, Pius² 

[1] Division of Rheumatology, University Hospital, CH-3010 Bern, Switzerland;Theodor-Kocher Institute, University of Bern, P.O. Box, CH-3000 Bern 9, Switzerland;Biomedical Research Centre and Department of Biochemistry, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada

关键词: Inflammation;    Chemotaxis;    Interleukin-8;    Receptor;    Transfection;    Jurkat cell;    IL-8R1/2;    human interleukin-8 receptor type 1/2;    J-IL8R1/2;    Jurkat transfectants expressing IL-8R1/2;    GROα and NAP-2 (neutrophil-activating peptide 2) are two chemotactic cytokines related to IL-8;    αCD3;    OKT3;    monoclonal antibody recognizing CD3 on T lymphocytes;   

DOI  :  10.1016/0014-5793(94)80454-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neutrophil leukocytes, the target cells for interleukin-8 and related CXC chemokines, bear high numbers of two types of IL-8 receptors (IL-8R1 and IL-8R2). By cDNA transfection Jurkat cell lines were generated that stably express either IL-8R1 or IL-8R2 (J-I8L8R1 and J-IL8R2). J-IL8R1 expressed 4,000 ± 1,000 copies of IL-8R 1, and bound IL-8 with high affinity (K _d 1–4 nM) and GROα and NAP-2 with low affinity (K _d 200–500 nM). J-IL8R2 expressed 17,000 ± 3,000 copies of IL-8R2, and bound all three chemokines with high affinity. Both transfectants showed a similar degree of chemotactic migration after stimulation with IL-8, GROa and NAP-2. All three chemokines were equally potent as attractants of J-IL8R2, whereas IL-8 was 300 to 1,000-fold more potent than GROα or NAP-2 as attractant of J-IL8R1. The potencies, therefore, agree with the affinities of the ligands to IL-8R1 and IL-8R2. Our results demonstrate that both IL-8 receptors function independently, and mediate chemotaxis in response to IL-8 and other CXC chemokines.

【 授权许可】

Unknown