The molecular chaperone TF55

FEBS Letters

Ureña, Dionisio¹  Baumeister, Wolfgang³  Pfeifer, Günter³  Waldmann, Thomas³  Carrascosa, José L.⁴  Hegerl, Reiner³  Marco, Sergio⁴  Peters, Jürgen³  Sack-Kongehl, Hilde² 

[1] Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Madrid, Spain;Fritz-Haber-Institut of the Max-Planck-Gesellschaft, Berlin, Germany;Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany;Centro Nacional de Biotecnología (CSIC), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain

关键词: Molecular chaperone;    Rotational symmetry;    Thermophilic factor (TF55);   

DOI  :  10.1016/0014-5793(94)80447-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

TF55-like factor from Sulfolobus solfataricus was purified to homogeneity and analyzed by electron microscopy and image analysis to determine the symmetries of these particles. Three different procedures were used to analyze the electron micrographs: (1) fuzzy-set based classification of the particles according to their rotational power spectra; (2) multivariate statistical analysis based on singular value decomposition; (3) circular harmonic analysis. Averages obtained from the three methods show unequivocally that the TF55-like complex presents a 9-fold symmetry.

【 授权许可】

Unknown