Progesterone increases the production of tissue inhibitor of metalloproteinases‐2 in rabbit uterine cervical fibroblasts

FEBS Letters

Itoh, Yoshifumi²  Mori, Yo¹  Imada, Keisuke¹  Nagase, Hideaki²  Ito, Akira¹ 

[1] Department of Biochemistry, Tokyo College of Pharmacy, Horinouchi, Hachioji, Tokyo 192-03, Japan;Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160-7421, USA

关键词: Progesterone;    TIMP-2;    Gelatinase A;    Matrix metalloproteinase;    Uterine cervix;    MMP;    matrix metalloproteinase;    TIMP;    tissue inhibitor of metalloproteinases;    MEM;    Eagle's minimum essential medium;    LAH;    lactalbumin hydrolysate;    FBS;    fetal bovine serum;    PBS(-);    calcium and magnesium-free phosphate-buffered saline;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(94)80250-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rabbit uterine cervical fibroblasts in culture produces tissue inhibitor of metalloproteinases (TIMP)-l and TIMP-2. When cells were treated with physiological concentrations of progesterone, the production of two TIMPs increased, and essentially all TIMP-2 was found to be complexed with promatrix metalloproteinase 2 (proMMP-2)/progelatinase A. Progesterone did not modulate the production of proMMP-2 and resulted in the increased total amount of proMMP-2-TIMP-2 complex. These observations provide the first evidence that progesterone participates in maintaining the homeostasis of connective tissue matrix in uterine cervix by augmenting both TIMP-1 and TIMP-2 production along with the known suppressive effects on the proMMP-1 and proMMP-3 production.

【 授权许可】

Unknown