Oxidative DNA base damage and antioxidant enzyme activities in human lung cancer

FEBS Letters

Jaruga, Pawel³  Zastawny, Tomasz H.³  Skokowski, Jan¹  Olinski, Ryszard³  Dizdaroglu, Miral² 

[1] Department of Surgery, Medical School, 80-211 Gdansk, Poland;Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA;Department of Clinical Biochemistry, Medical School, 85-094 Bydgoszcz, Poland

关键词: Oxidative DNA damage;    Hydroxyl radical;    Mutation;    Modified base;    Antioxidant enzyme;    O2 −;    superoxide radical;    'OH;    hydroxyl radical;    5-OH-Hyd;    5-hydroxyhydantoin;    5-OH-5-MeHyd;    5-hydroxy-5-methylhydantoin;    Thy glycol;    thymine glycol;    5-OHMe-Ura;    5-(hydroxymethyl)uracil;    5-OH-Ura;    5-hydroxyuracil;    5-OH-Cyt;    5-hydroxycytosine;    5;    6-diOH-Ura;    5;    6-dihydroxvuracil;    2-OH-Ade;    2-hydroxyadenine;    8-OH-Ade;    8-hydroxyadenine;    FapyAde;    4;    6-diamino-5-formamidopyrimidine;    8-OH-Gua;    8-hydroxyguanine;    FapyGua;    2;    6-diamino-4-hydroxy-5-formamidopyrimidine;    GC/MS-SIM;    gas chromatography-mass spectroscopy with selected-ion monitoring;    GPx;    glutathioneperoxidase;    SOD;    superoxide dismutase;    CAT;    catalase;   

DOI  :  10.1016/0014-5793(94)80240-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated levels of antioxidant enzymes and free radical-induced DNA base modifications in human cancerous lung tissues and in their cancer-free surrounding tissues. Various DNA base lesions in chromatin of lung tissues were measured by gas chromatography-mass spectrometry. Activities of superoxide dismutase, catalase and glutathione peroxidase were also measured in lung tissues. Higher levels of DNA lesions were observed in cancerous tissues than in cancer-free surrounding tissues. Antioxidant enzyme levels were lower in cancerous tissues. The results indicate an association between decreased activities of antioxidant enzymes and increased levels of DNA lesions in cancerous tissues. Higher levels of DNA lesions suggest that free radical reactions may be increased in malignant tumor cells.

【 授权许可】

Unknown