Differential influence of DNA supercoiling on in vivo strength of promoters varying in structure and organisation in E. coli

FEBS Letters

Mishra, Rakesh K.¹  Jyothirmai, G.¹ 

[1] Centre for Cellular and Molecular Biology, Hyderabad 500 007, India

关键词: Transcription;    Promoter;    DNA supercoiling;    Escherichia coli;   

DOI  :  10.1016/0014-5793(94)80135-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA supercoiling is known to influence promoter activity in vitro and in vivo in a promoter-dependent manner in prokaryotes. In order to investigate how topology may influence promoter function, we have studied two kinds of promoter variants, (i) where only the spacer region is altered, and (ii) where the same promoter is tandemly repeated in either the same or opposite orientation. These promoters respond very differently to alterations in DNA supercoiling, suggesting that the overall structure of the promoter and its context contribute to the differential response to alterations in supercoiling in vivo.

【 授权许可】

Unknown