Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein

FEBS Letters

Taniguchi, Shigehiko¹  Fujita, Nobuyuki³  Nakanishi, Tohru¹  Ishihama, Akira³  Nohno, Tsutomu²  Hattori, Takako¹  Takahashi, Kojiro¹ 

[1] Department of Biochemistry, Okayama University Dental School, Okayama 700, Japan;Department of Pharmacology, Kawasaki Medical School, Kurashiki 701-01, Japan;Department of Molecular Genetics, National Institute of Genetics, Mishima 411, Japan

关键词: Transcriptional regulation;    FNR;    fnr;    nar X;    Upstream activation signal;    Escherichia coli;   

DOI  :  10.1016/0014-5793(94)80173-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In facultative anaerobes, the anaerobic expression of respiratory genes is regulated by a transcriptional activator, FNR. Transcription in vitro of the E. coli fnr gene was repressed by its product, FNR. The transcription of the E. coli nar X gene encoding the nitrate sensor protein was likewise repressed. DNA truncation experiments for fnr and nar X genes indicated that multiple anaero-boxes in each promoter region are essential for repression by the FNR protein, but they also suggest that factor-independent upstream activation signals are operating with these promoters.

【 授权许可】

Unknown