pH‐dependent formation of ethylenediaminetetraacetic acid supramolecular aggregates

FEBS Letters

Müller, Michele¹  Haeberli, André¹ 

[1] Department of Medicine, Laboratory for Thrombosis Research Inselspital, CH-3010 Bern, Switzerland

关键词: Fibrin;    γ-Chain-petide;    Polymerization;    Ethylenediaminetetraacetic acid;    Supramolecular aggregate;    EDTA;    Ethylenediaminetetraacetic acid;    EGTA;    Ethylene glycol-O;    O'-bis(2-aminoethyl)-N;    N;    N';    N'-tetraacetic acid;    HEPES;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    M r;    molecular mass;    Tris;    tris(hydroxymethyl)aminomethane;   

DOI  :  10.1016/0014-5793(94)80164-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pure ethylenediaminetetraacetic acid tetrasodium salt (EDTA · Na₄) in water showed a pH-dependent change of optical density detected in the ultraviolet region. The most important change was found between pH 4 and 8, corresponding to the deprotonation of the third ionizable group having a pK of 6.2. The absorbance change was reversible and depended on the addition/removal of only 1 proton equivalent. The presence of supramolecular aggregates was suggested by size-exclusion chromatography, dialysis and cryo-electronmicroscopy. The molecular weight of EDTA supramolecular aggregates was 10–15,000 and 8–14,000, as determined by gel chromatography and dialysis, respectively. Cryo-electronmicroscopy revealed structures of 2–4 nm in diameter.

【 授权许可】

Unknown