FEBS Letters | |
Phospholipase A2 enhances [3H]AMPA binding to a putative homomeric GluR‐B receptor in the rat spinal cord | |
Henley, Jeremy M.1  Cruickshank, Alison M.1  | |
[1] Department of Pharmacology, Medical School, University of Birmingham, Edgbaston, Birmingham, BIS 2TT, England | |
关键词: Spinal cord; GluR-B; AMPA; Phospholipase A2; Kainate; Autoradiography; | |
DOI : 10.1016/0014-5793(94)80408-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
[3H]AMPA and [3H]kanine binding to rat spinal cord was localised most densely in the substantia gelatinosa of the dorsal horn. Phospholipase A2 elicited a dose-dependent increase in specific [3H]AMPA binding but not in [3H]kainate binding. The enhancement of [3H]AMPA binding was blocked by bromophenacyi bromide and at 0'C and was not mimicked by arachidonic acid. Since GluR-B is the only AMPA receptor subunit detectable in the rat spinal cord, and recombinant homomeric GluR-B assemblies do not bind [3H]kainate, these results suggest phospholipase A2 may modulate [3H]AMPA binding to putative homomeric GluR-B receptors.
【 授权许可】
Unknown
【 预 览 】
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