Identification of a chaperonin‐10 homologue in plant mitochondria

FEBS Letters

Leaver, C.J.¹  Burt, W.J.E.¹ 

[1] Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK

关键词: Molecular chaperone;    Chaperonin;    Plant mitochondrion;    ATP;    adenosine 5'-triphosphate;    BSA;    bovine serum albumin (fraction V);    EGTA;    ethyleneglycol bis-(β-aminoaethyl ether) N;    N;    N';    N'-tetrnsicetic acid;    HPLC;    high-performance liquid chromatography;    MOPS;    3-(N-morpholino)propanesulphonic acid;    PMSF;    phanylmethylsulfonyl fluoride;    PAGE;    polyacrylamide gel electrophoresis;    PTH;    phenylthiohydantoin;    Rubisco;    ribulose 1;    5-bisphosphate carboxylase/oxygenase;    SDS;    sodium dodecyl sulphate;   

DOI  :  10.1016/0014-5793(94)80401-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chaperonin-60 and chaperonin-10 form stable binary complexes in the presence of ATP. This phenomenon has been used as the basis for the identification of a chaperonin-10 homologue in potato (Solanum tuberosum L.) mitochondria. ATP-dependent binary complexes formed between chaperonin-60 and chaperonin-10 in potato mitochondrial extracts were isolated by affinity chromatography on a column of immobilised chaperonin-60 antibodies. Partial amino acid sequence analysis of the chaperonin-10 protein indicates that it is related in primary structure to rat mitochondrial chaperonin-10.

【 授权许可】

Unknown