Role of type II phospholipase A2 in the inflammatory process of carrageenan‐induced pleurisy in rats

FEBS Letters

Inoue, Keizo¹  Murakami, Makoto¹  Okamoto, Hitoshi²  Kakutani, Makoto²  Kudo, Ichiro¹ 

[1] Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan;Japan Tobacco Inc., Pharmaceutical Research Laboratories, 1-1, Murasakicho, Takatuki, Osaka 569, Japan

关键词: Phospholipase A2;    Carrageenan-induced pleurisy;    Inflammation;   

DOI  :  10.1016/0014-5793(94)80388-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to investigate the role of type II phospholipase A₂ (PLA₂) in the inflammatory process, the effect of a monoclonal antibody specific to type II PLA₂ on carrageenan-induced pleurisy was studied in rats. Intravenous injection of the antibody (MB5.2), which inhibits the catalytic activity of type II PLA₂, significantly reduced both the pleural exudate volume and the intrapleural leukoeyte count, while a control antibody did not have any appreciable effect. MB5.2 caused no change in the level of type II PLA₂ in the pleural fluid. These results suggest that type II PLA₂ generated at inflamed sites, at least in part, have a crucial role in the pathogenesis of acute inflammation.

【 授权许可】

Unknown