FEBS Letters | |
Tris: an allosteric effector of tarantula haemocyanin | |
Decker, H.1  Bardehle, K.1  Sterner, R.1  Paul, R.1  | |
[1] Zoologisches Institut der Universität München, Luisenstr. 14, D-80333 München 2, Germany | |
关键词: Allostery; Chloride effect; Haemocyanin; Nesting model; Oxygen binding; Tarantula; Tris; | |
DOI : 10.1016/0014-5793(94)80379-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The effect of the chemical buffering component Tris (hydroxy-methyl-amino-methane) and of chloride ions on the oxygen binding of tarantula hemocyanin was studied at constant pH. It revealed that Tris at micromolar concentrations decreases the oxygen pressure at half-saturation (P50) by a factor of more than two, whereas chloride does not influence oxygen affinity. A thermodynamic analysis in terms of the nested model of allostery [(1987) Proc. Natl. Acad. Sci. 84, 1891-1895] indicated that Tris acts a an allosteric activator of oxygen binding by influencing the interaction between the 12-meric half-molecules of the 24-meric tarantula haemocyanin.
【 授权许可】
Unknown
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