Cloning of the classical guinea pig pancreatic lipase and comparison with the lipase related protein 2

FEBS Letters

Carrière, Frédéric¹  Hjorth, Siv¹  Thirstrup, Kenneth¹  Boel, Esper¹ 

[1] Novo Nordisk, Novo allé, DK-2880 Bagsvœrd, Denmark

关键词: Colipase;    Polymerase chain reaction;    Phospholipase;    Phospholipid;    Triglyceride;   

DOI  :  10.1016/0014-5793(94)80117-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Starting from total pancreatic mRNAs, the classical guinea pig pancreatic lipase was cloned using rapid amplification of 3' and 5' cDNA ends. Internal oligonucleotide primers were designed from a partial cDNA clone including the region coding for the lid domain. Using this strategy, we did not amplify the cDNA corresponding to the pancreatic lipase related protein 2 in which the lid domain is deleted. Amino acid sequences of the classical guinea pig pancreatic lipase and the related protein 2 were compared based on the primary and tertiary structures of the classical human pancreatic lipase. Their distinct physiological roles are discussed in the light of functional amino acid differences.

【 授权许可】

Unknown