Characterisation of the F‐actin binding domains of villin: classification of F‐actin binding proteins into two groups according to their binding sites on actin

FEBS Letters

Way, Michael¹  Matsudaira, Paul T.¹  Pope, Brian²  Weeds, Alan² 

[1]Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142, USA

[2]MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

关键词: Villin;    Gelsolin;    Actin depolymerizing factor;    Actin-binding protein;    ADF;    actin depolymerizing factor from human brain;    V2-3;    segments 2 and 3 of chicken villin;    G2;    segment 2 of human gelsolin;   

DOI  :  10.1016/0014-5793(94)80116-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The F-actin binding properties of chicken villin, its headpiece and domains 2–3 (V2-3) have been analysed to identify sites involved in bundle formation. Headpiece and V2-3 bind actin with K _d values of ~7 μM and ~0.3 μM, respectively, at low ionic strength. V2-3 binding, like that of villin, is weakened with increasing salt concentration; headpiece binding is not. Competition experiments show that headpiece and V2-3 bind to different sites on actin, forming the two cross-linking sites of villin. Headpiece does not compete with the F-actin binding domains of gelsolin or α-actinin, but it dissociates actin depolymerizing factor. We suggest that the F-actin binding domains of actin severing, crosslinking and capping proteins can be organized into two classes.

【 授权许可】

Unknown