Positive and negative regulatory elements are involved in transcriptional control of the rat glucokinase gene in the insulin producing cell line HIT M2.2.2

FEBS Letters

Leibiger, Barbara¹  Leibiger, Ingo B.¹  Pett, Ute¹  Walther, Reinhard¹ 

[1] Institut für Biochemie, Klinikum, Medizinische Fakultät, Ernst-Moritz-Arndt-Universität Greifswald, Fleischmannstraße 42-44, D-17489 Greifswald, Germany

关键词: Glucokinase;    Gene expression;    Pancreatic β-Cell;    DNA-protein interaction;    Promoter;   

DOI  :  10.1016/0014-5793(94)80265-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nested deletion mutants of the 5' flanking region of the β-cell transcription unit of the rat glucokinase gene (rßGK) were fused to the CAT-reporter gene. Transient expression studies in HIT M2.2.2 and BHK21 cells revealed a distal (upstream of −359) and a proximal promoter region (between −278/−49) harbouring positive and negative regulatory elements. DNaseI footprinting revealed three protected areas between nucleotides −190 and −60. DNA-elements playing a crucial role in transcriptional control of the insulin genes (IEB- and CT-motifs) have been detected within the proximal promoter region and contribute to β-cell specific gene regulation. 3' deletion analysis revealed that DNA-elements located downstream from transcription initiation sites (up to +123) contribute to transcriptional regulation.

【 授权许可】

Unknown