Cloning and sequence analysis of a cDNA encoding rice glutaredoxin

FEBS Letters

Tanaka, Kunisuke¹  Ichihara, Ken'ichi¹  Masumura, Takehiro¹  Yabushita, Tetsushige¹  Minakuchi, Kazunobu¹ 

[1] Department of Biochemistry, College of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto 606, Japan

关键词: Glutaredoxin;    Thioltransferase;    Aleurone layer;    Rice;    Oryza sativa L;   

DOI  :  10.1016/0014-5793(94)80264-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full-length cDNA clone (RASC8) encoding glutaredoxin (thioltransferase) was isolated from a cDNA library of an aleurone layer prepared from a developing seed of rice (Oryza sativa L.). RASC8, 568bp in length, contained an ATG codon and two possible polyadenylation signals, and encoded 112 amino acid residues. Cys-Pro-Phe-Cys, which is the active site and a highly conserved sequence among thioltransferases, was found in the deduced amino acid sequence. RASC8 was introduced into an expression vector pMALc2 and the translated product possessed thioltransferase activity.

【 授权许可】

Unknown