Ionic channel activity induced by fusion of Rhodospirillum rubrum chromatophores with a planar bilayer lipid membrane

FEBS Letters

Antonenko, Yuri N.¹  Kotova, Elena A.¹  Rokitskaya, Tatiana I.¹  Taisova, Aleksandra S.¹ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation

关键词: Ionic channel;    Chromatophore membrane;    Planar lipid bilayer;    Rhodospirillum rubrum;   

DOI  :  10.1016/0014-5793(94)80633-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present work concerns mechanisms of ionic conductivity of photosynthetic membranes. It is shown that reconstitution of vesicles of photosynthetic membranes (chromatophores) of purple bacteria Rhodospirillum rubrum into a planar bilayer lipid membrane leads to fluctuations of current showing the existence of a channel with a predominant conductance of ~ 230 pS in the presence of 100 mM KCl. Measurements under the conditions of KCl gradient prove that this channel is cation selective (P _k/P _cl = 7.2). Voltage inactivation of the channel is demonstrated which is prevented by treatment with trypsin.

【 授权许可】

Unknown