γ‐Glutamylcysteinylglutamic acid ‐ a new homologue of glutathione in maize seedlings exposed to cadmium

FEBS Letters

Meuwly, Philippe¹  Rauser, Wilfried E.¹  Thibault, Pierre² 

[1] Department of Botany, College of Biological Sciences, University of Guelph, Guelph, Ont. N1G2W1, Canada;Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, NS, B3H3Z1, Canada

关键词: γ-Glutamylcysteinylglutamic acid;    Glutathione;    Metal detoxification;    Cd-binding peptide;    Phytochelatin;    MS-MS;    (γEC)nG;    phytochelatins;    γEC;    γ-glutamylcysteine;    γECG;    γ-glutamylcysteinylglycine (glutathione);    γECßA.;    γ-glutamylcysteinyl-β-alanine;    (γEC)nβA;    homo-phytochelatins;    γECS;    γ-glutamylcysteinylserine;    MS-MS;    tandem mass spectrometry;    γECE;    γ-glutamylcysteinylglutamic acid;    ACN;    acetonitrile;    TFA;    trifluoroacetic acid;    DTNB;    5;    5'-dithiobis(2-nitrobenzoic acid);    TNB;    thiobis-(2-nitrobenzoic acid);    DMF;    dimethyl formamide;   

DOI  :  10.1016/0014-5793(93)80858-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of plants to Cd induces the appearance of several thiols based on glutathione and known as class III metallothioneins (or phytochelatins). A new tripeptide with the structure γ-GluCysGlu accumulated in roots and shoots of Cd-exposed maize seedlings. This thiol was purified and identified by tandem mass spectrometry. The fragmentation pattern of the maize tripeptide was identical to that of the synthetic compound. Like glutathione, this new tripeptide may serve as a precursor for longer-chain peptides involved in metal detoxification through the formation of Cd-binding complexes.

【 授权许可】

Unknown