Synchronous replication of SV 40 DNA in virus infected TC 7 cells induced by transient hypoxia

FEBS Letters

Probst, Hans²  Dreier, Torsten²  Scheidtmann, Karl Heinz¹ 

[1] Institut für Genetik, Abteilung Molekulargenetik der Universität Bonn, D-53117 Bonn, Germany;Physiologisch-chemisches Institut der Universität Tübingen, Hoppe-Seyler-Straße 4, D-72076 Tübingen, Germany

关键词: DNA replication;    Replicon initiation;    Synchronisation of replication;    Hypoxia;    Simian virus 40;   

DOI  :  10.1016/0014-5793(93)80853-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We transiently exposed SV 40 infected TC 7 cell cultures to a reduced O₂ tension (4–8 h, about 200 ppm relative to 10⁵ Pa total pressure). Under the hypoxic conditions, ‘working’ viral replication forks were greatly retarded or stopped, and initiation of daughter strand synthesis in further SV 40 DNA molecules was suppressed. Reoxygenation released an immediate burst of SV 40 replication which mainly consisted of a synchronous viral replication round. This synchronous in vivo replication began at the known origin of replication and proceeded at normal rates to the known termination region. Viral replicons seemed to accumulate under hypoxia in a state fully prepared to begin replication immediately after recovery of a normal pO₂. The shut-down and sudden reactivation of DNA synthesis under hypoxia and reoxygenation, respectively, were not accompanied by changes of the phosphorylation state of large T antigen. The described synchronization procedure can be applied to optionally large SV 40 infected cell cultures.

【 授权许可】

Unknown