Modulation of mammalian cell proliferation by a modified tRNA base of bacterial origin

FEBS Letters

Reisser, Thomas¹  Kersten, Helga¹  Langgut, Werner¹  Nishimura, Susumu² 

[1] Institut für Biochemie der Medizinischen Fakultät, Universität Erlangen-Nürnberg, Fahrstrasse 17, D-91054 Erlangen, Germany;Biology Division, Natlonal Cancer Center Research Insitute, Tsukiji-5-chome, chuo-ku, Tokyo, Japan

关键词: Queuine;    Cell proliferation;    Hypoxia;    Oncogenic transformation;    tRNA modification;    Aerobic glycolysis;   

DOI  :  10.1016/0014-5793(93)81627-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of the q-base to q-deflcient non-transformed mammalian cells stimulated their proliferation. The q-base also improved proliferation of some cancer-derived cell lines, but inhibited proliferation of others. The proliferation of HeLa-S3 carcinoma cells was stimulated by q under aerobic conditions, but was inhibited when the cells had shifted their energy metabolism towards glycolysis as the result of oxygen limitation. Q-deficient cells could not adapt their proliferation to the respective oxygen tension. The q-base stimulated the proliferation of non-transformed fibroblasts but inhibited proliferation of the same cell line, when aerobic glycolysis was increased after transformation with the ras gene. The results suggest that the q-base permits mammalian cells to adapt their proliferation to their specific metabolic state.

【 授权许可】

Unknown