A mutation causing constitutive synthesis of the pyruvate dehydrogenase complex in Escherichia coli is located within the pdhR gene

FEBS Letters

Haydon, David J.¹  Quail, Michael A.¹  Guest, John R.¹ 

[1] The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, S10 2UH, UK

关键词: Pyruvate dehydrogenase complex;    Transcriptional regulation;    PdhR regulatory protein;    Gene expression;    Escherichia coli;    PDH;    pyruvate dehydrogenase;    Elp;    pyruvate dehydrogenase (decarboxylating);    E2p;    lipoate acetyltransferase;    E3;    lipoamide dehydrogenase;   

DOI  :  10.1016/0014-5793(93)81605-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The aceE-aceF-lpd genes encoding the pyruvate dehydrogenase (PDH) complex of Escherichia coli are preceded by a gene encoding a putative transcriptional regulator, PdhR (formerly designated Gen A). Enzymological tests and studies with pdhR-lacZ and aceE-lacZ translational fusions have shown that a constitutive mutation (ace^c816), which increases PDH complex synthesis to the pyruvate-induced level in the absence of inducer, is recessive to the wild-type pdhR gene in trans. Sequence comparisons further showed that the ace^c816 mutation affects a single site in the pdhR gene leading to an Arg¹¹⁸ (CGU) → Cys (UGU) substitution in the PdhR protein. The results support the view that synthesis of the PDH complex is regulated from the pdhR promoter of a pdhR-aceEF-lpd operon.

【 授权许可】

Unknown