Expression of the alpha subunit of PABA peptide hydrolase (EC 3.4.24.18) in MDCK cells

FEBS Letters

Dumermuth, Eric¹  Grünberg, Jürgen¹  Eldering, Joyce A.¹  Sterchi, Erwin E.¹ 

[1] Institute of Biochemistry and Molecular Biology, University of Berne, Bühlstrasse 28, CH-3012 Berne, Switzerland

关键词: PABA peptide hydrolase;    Astacin;    Meprin;    Intestine;    MDCK;    Processing;    PPH;    PABA peptide hydrolase;    PPHα;    alpha subunit of PABA peptide hydrolase;    meprina;    alpha subunit ofmeprin;    FITC;    fluorescein isothiocyanate;   

DOI  :  10.1016/0014-5793(93)80422-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this paper, we report the expression of PPHα in the polarized cell line MDCK (Madin Darby canine kidney). In these cells, the enzyme was synthesized m an inactive profonn, which upon treatment with trypsin was activated. The enzyme isolated from cell extracts was core-glycosylated and appeared to be retained in the ER as a homodimer. No PPHα was detectable on the surface of intact cells by immunofluoreseence. However, a complex glycosylated soluble but inactive form was present in the culture medium, suggesting that proteolytic removal of the C-terminal membrane anchoring peptide leads to the secretion of PPHα.

【 授权许可】

Unknown