Molecular characterization of Phl p II, a major timothy grass (Phleum pratense) pollen allergen

FEBS Letters

Steinberger, Peter¹  Dolecek, Christiane¹  Scheiner, Otto¹  Kraft, Dietrich¹  Valenta, Rudolf¹  Vrtala, Susanne¹  Laffer, Sylvia¹ 

[1] Institute of General and Experimental Pathology, AKH, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria

关键词: Type I allergy;    cDNA cloning;    Grass pollen allergen;    Phl p II;   

DOI  :  10.1016/0014-5793(93)80406-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Grass pollen allergens belong to the most important and widespread elicitors of pollen allergy. Using serum IgE from a grass pollen allergic patient, a complete cDNA encoding a group II allergen was isolated from a timothy grass (Phleum pratense) pollen expression library. The deduced amino acid sequence of the Phl p II allergen shows an average sequence identity of 61% with the protein sequences determined for group II/III allergens from rye grass (Lolium perenne) and a sequence identity of 43% with the C-terminal portion of group I grass pollen allergens from different species. A hydrophobic leader peptide similar to leader peptides found in other major grass pollen allergens heads the deduced amino acid sequence, indicating that group II/III grass pollen allergens belong to a family of secreted proteins. Serum IgE specific for Phl p II, detected the protein exclusively in pollen and not in other plant tissues. The recombinant Phl p II was expressed in Escherichia coli and showed similar IgE-binding capacity as the natural allergen.

【 授权许可】

Unknown