Suppression of neuronal potassium A‐current by arachidonic acid

FEBS Letters

Villarroel, Alvaro¹ 

[1] Howard Hughes Medical Institute, Department of Neurobiology and Behavior, SUNY at Stony Brook, Stony Brook, NY11794-5230, USA

关键词: A-current;    M-current;    Potassium current;    Inhibition;    Arachidonic acid;    Sympathetic neuron;   

DOI  :  10.1016/0014-5793(93)80726-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of arachidonic acid on the A current (I _A) has been studied in dissociated bullfrog neurons under whole-cell voltage-damp conditions. Arachidonic acid reduced I _A in a dose-dependent and reversible manner without a shift in the prepulse inactivation voltage-current relation. 1.75 μM inhibited i _A by 50%, and higher concentrations caused a total suppression. In addition, arachidonic add increased the M-current (I _M), a different potassium current that does not inactivate. Neither indomethacin nor nordihydroguaiaretic acid, cyclooxygenase and lipoxygenase inhibitors respectively, prevented _{I A} reduction. In contrast, nordihydroguaiaretic acid prevented I _M enhancement. Eicosatetraynoic acid, an arachidonic acid analog that cannot be metabolized, also reduced I _A. These results suggest that arachidonic acid metabolism is not required to suppress I _A

【 授权许可】

Unknown