RPII15 codes for the M r 15,000 subunit 9 of Drosophila melanogaster RNA polymerase II

FEBS Letters

Bautz, Ekkehard K.F.¹  Kontermann, Roland E.¹  Liu, Zhihong¹  Schulze, Rüdiger A.¹  Petersen, Gabriele¹ 

[1] Institute of Molecular Genetics, University of Heidelberg, Im Neuenheimer Feld 230, D-69120 Heidelberg, Germany

关键词: RNA polymerase II;    Small subunit;    Maltose-binding protein;    Fusion protein;    Drosophila melanogaster;    CTD;    C-terminal heptapeptide repeat domain;    MBP;    maltose-binding protein;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(93)80442-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The RPII15 gene product of Drosophila melanogaster, which has recently been identified by sequence comparison, possesses a high similarity to subunit 9 of yeast RNA polymerase II. Using the polymerase chain reaction the coding region of RPII15 was isolated from genomic DNA of adult flies. Sequence analysis shows four amino acid substitutions in comparison to the previously reported sequence. Antisera were generated against bacterially expressed RPII15 and were used for immunoblotting experiments with RNA polymerase II of Drosophila melanogaster. This analysis identified the M _r, 15,000 subunit 9 as gene product of RPII15.

【 授权许可】

Unknown