Immediate‐early gene induction by repetitive mechanical but not electrical activity in adult rat cardiomyocytes

FEBS Letters

Wollnik, Bernd¹  Maass, Alexander¹  Kubisch, Christian¹  Neyses, Ludwig¹  Vetter, Hans¹  Meyer, Rainer² 

[1] Medical Policlinic, University of Bonn, Wilhelmstr. 35-37, 5300 Bonn 1, Germany;Institute of Physiology, University of Bonn, 5300 Bonn 1, Germany

关键词: Heart;    egr-1;    c-fos;    Contraction;    2;    3-Butanedione monoxime;   

DOI  :  10.1016/0014-5793(93)80434-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mechanical factors are thought to play an important role in the induction of myocardial hypertrophy. Yet, it is not known whether active contraction induces genes that probably represent initial steps in the hypertrophic response in the adult myocardium - and if so, whether the mechanical or the electrical component of the twitch governs this response. We therefore investigated whether electrical stimulation of contraction was able to induce the immediate-early genes (IEGs) egr-1 and c-fos in adult rat cardiomyocytes. Cyclical contraction led to an increase in egr-1 and c-fos mRNA levels within 30 min. Full inhibition of contraction during electrostimulation by the Ca²⁺-desensitizer 2,3-butanedione monoxime (BDM) totally blocked this IEG-response without altering membrane potential. These data suggest that in adult myocardium, the mechanical rather than the electrical activity is responsible for the IEG-response during active twitch.

【 授权许可】

Unknown