Effect of hyperthermia on the extracellular matrix I. Heat enhances hyaluronan and inhibits sulphated glycosaminoglycan synthesis

FEBS Letters

Wegrowski, Yanusz¹ 

[1] Laboratoire de Biochimie, CNRS ERS 0017, Faculté de Médecine, Université de Reims, 51095 Reims Cedex, France

关键词: Hyperthermia;    Heat shock;    Glycosaminoglycan;    Hyaluronan;    Inflammation;   

DOI  :  10.1016/0014-5793(93)81695-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The synthesis of sulphated glycosaminoglycans (GAG) and hyaluronan (HA) was studied in 3 cell strains incubated at 37°C or 42°C. Cells were labelled with [³H]glucosamine and [³⁵S]sulphate. No incorporation of [³⁵S]sulphate was observed at 42°C. Cellulose acetate electrophoresis of GAGs synthetized at 42°C showed only one [³H]glucosamine-labelled band completely digested with Streptomyces hyaluronidase. The quantification of [³H]glucosamine-labelled GAGs before and after hyaluronidase treatment indicated an increased rate of HA synthesis at 42°C as compared to the rate of synthesis at 37°C. These results suggest that hyperthermia stimulates HA synthesis and inhibits sulphated GAG synthesis in several cell strains which in turn may contribute to the modification of extracellular environment in inflammation.

【 授权许可】

Unknown