The three‐dimensional structure of the colicin E3 immunity protein by distance geometry calculation

FEBS Letters

Yajima, Shunsuke²  Morikawa, Souichi¹  Nakamura, Haruki¹  Yokoyama, Shigeyuki³  Masaki, Haruhiko²  Uozumi, Takeshi²  Muto, Yutaka³ 

[1] Protein Engineering Research Institute, Furuedai, Suita, Osaka 565, Japan;Department of Biotechnology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113, Japan;Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Nuclear magnetic resonance;    Colicin E3;    Distance geometry;    Protein-protein interaction;    NMR;    nuclear magnetic resonance;    NOE;    nuclear Overhauser effect;   

DOI  :  10.1016/0014-5793(93)80665-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The three-dimensional solution structure of the colicin E3 immunity protein (84 residues) was determined by distance geometry calculations. The hydrophilic side of a four-stranded antiparallel β-sheet constitutes a part of the surface of the protein, and two loops lie on the hydrophobic side of the sheet. All the three specificity-determining residues, which are included in the center of the β-sheet, display their side groups on the protein surface.

【 授权许可】

Unknown