Reversible inactivation of endothelial nitric oxide synthase by N G‐nitro‐l‐arginine

FEBS Letters

Klatt, Peter¹  Schmidt, Kurt¹  Schmid, Martin¹  Mayer, Bernd¹ 

[1] Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, A-8010 Graz, Austria

关键词: Endothelial cell;    Nitric oxide synthase;    Inhibitor;    Inactivation;    L-Arginine;    PAEC;    porcine aortic endothelial cells;    NOS;    NO synthase;    L-NMA;    N G-methyl-l-arginine;    L-NNA;    N G-nitro-l-arginine;    IC50;    concentration producing 50% inhibition;   

DOI  :  10.1016/0014-5793(93)80405-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N ^G-Methyl-l-arginine (L-NMA) and N ^G-nitro-l-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 μM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by wash-out in the presence of 1 mM l-arginine. In short-term incubations (45 s) of membrane fractions, L-NMA and L-NNA exhibited similar potencies to inhibit endothelial NO synthase, but L-NNA was markedly more potent than L-NMA after prolonged incubation periods (⩾ 3 min) due to induction of a pronounced, reversible enzyme inactivation.

【 授权许可】

Unknown