New virus‐specific T‐helper epitopes of foot‐and‐mouth disease viral VP1 protein

FEBS Letters

Surovoy, Andrey Yu.¹  Volpina, Olga M.¹  Yarov, Aleksey V.¹  Chepurkin, Anatoly V.¹  Gelfanov, Vasily M.¹  Ivanov, Vadim T.¹ 

[1] Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10 117871 GSP Moscow, Russian Federation

关键词: Foot-and-mouth disease;    Synthetic peptide immunogenicity;    T-cell recognition site;    PAM;    4-(oximethyl)phenylacetamidomethyl;    TPA;    titer of antipeptide antibodies;    TVA;    titer of virus neutralizing antibodies;    CFA;    complete Freund's adjuvant;    IFA;    incomplete Freund's adjuvant;   

DOI  :  10.1016/0014-5793(93)80399-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunogenicity studies of synthetic peptides from different regions of VP₁ protein of foot-and-mouth disease virus strain A₂₂ revealed the following active fragments: 39–61, 50–69, 135–159, 175–189, 170–189 and 197–213. Testing of virus neutralizing antibody production in rabbits primed by peptides and then inoculated by the virus showed that only peptides 135–159 and 170–189 were able to induce the functional T-cell helper activity. Localization of virus-specific T-cell recognition sites in sequences 135–159 and 170–189 was confirmed in in vitro recognition experiments of the virus by peptide activated mice lymphocytes.

【 授权许可】

Unknown