Direct gene transfer into mouse diaphragm

FEBS Letters

Jasmin, Bernard J.¹  Davis, Heather L.² 

[1] Department of Physiology, Faculty of Medicine, University of Ottawa, Ottawa, K1H 8M5, Canada;Physiotherapy Program, Faculty of Health Sciences, University of Ottawa, 451 Smyth Road, Ottawa, K1H 8M5, Canada

关键词: Gene transfer;    Muscle;    Diaphragm;    Muscular dystrophy;   

DOI  :  10.1016/0014-5793(93)80393-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Direct gene transfer into skeletal muscle is a potential therapeutic strategy for inherited primary myopathies such as Duchenne muscular dystrophy (DMD). In order to affect the life-expectancy of these patients, it will be necessary to carry out gene therapy on the diaphragm. To this end, we report efficient introduction of pure recombinant plasmid DNA into the mouse diaphragm, without causing significant damage. Application of this approach to the diaphragm of the mdx mouse will provide information on the potential usefulness of gene therapy for the treatment of DMD patients.

【 授权许可】

Unknown