The pro sequence of lactase‐phlorizin hydrolase is required for the enzyme to reach the plasma membrane

FEBS Letters

Mantei, Ned¹  Oberholzer, Thomas¹  Semenza, Giorgio¹ 

[1] Department of Biochemistry, Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zürich, Switzerland

关键词: Sorting;    Targeting;    Pro sequence;    Lactase-phlorizin hydrolase;    Plasma membrane;    Proteolytic processing;   

DOI  :  10.1016/0014-5793(93)80389-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Various cDNAs coding for part or all of human pre-pro lactase-phlorizin hydrolase (pre-proLPH) were transfected into COS cells and the subcellular location of the lactase-related proteins assessed. Only the complete proLPH reached the plasma membrane. LPH without the pro sequence, and a construct containing the pro sequence and the lactase domain of mature LPH, accumulated intracellularly; the pro sequence with no mature domain was secreted. We conclude that the pro sequence is important for LPH to be transported to the cell surface.

【 授权许可】

Unknown