Trimeric mutant bacteriorhodopsin, D85N, shows a monophasic CD spectrum

FEBS Letters

Lanyi, Janos K.¹  Mihara, Ken'ichi²  Tokunaga, Fumio²  Needleman, Richard³  Kamikubo, Hironari⁴  Kataoka, Mikio² 

[1] Department of Physiology and Biophysics, University of California, Irvine, CA 92717, USA;Department of Biology, Faculty of Science, Osaka University, Toyonaka 560, Japan;Department of Biochemistry, Wayne State University School of Medicine, Detroit, MI 48201, USA;Department of Physics, Faculty of Science, Osaka University, Toyonaka 560, Japan

关键词: Bacteriorhodopsin;    Circular dichroism;    Exciton coupling;    Site-specific mutant;    X-ray diffraction;   

DOI  :  10.1016/0014-5793(93)80385-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The structure of mutant bacteriorhodopsin (bR), D85N, was examined by CD and X-ray diffraction at pH 7. The absorption maximum of D85N at pH 7 is located at 605 nm, which is similar to the acid-blue form of wild-type bR. D85N shows a monophasic CD band, the maximum of which is at 575 nm, although the crystalline arrangement and the trimeric structure is maintained. The acid-blue form of wild-type bR shows a biphasic CD despite the similarity in absorption spectra.

【 授权许可】

Unknown