期刊论文详细信息
FEBS Letters | |
Trimeric mutant bacteriorhodopsin, D85N, shows a monophasic CD spectrum | |
Lanyi, Janos K.1  Mihara, Ken'ichi2  Tokunaga, Fumio2  Needleman, Richard3  Kamikubo, Hironari4  Kataoka, Mikio2  | |
[1] Department of Physiology and Biophysics, University of California, Irvine, CA 92717, USA;Department of Biology, Faculty of Science, Osaka University, Toyonaka 560, Japan;Department of Biochemistry, Wayne State University School of Medicine, Detroit, MI 48201, USA;Department of Physics, Faculty of Science, Osaka University, Toyonaka 560, Japan | |
关键词: Bacteriorhodopsin; Circular dichroism; Exciton coupling; Site-specific mutant; X-ray diffraction; | |
DOI : 10.1016/0014-5793(93)80385-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The structure of mutant bacteriorhodopsin (bR), D85N, was examined by CD and X-ray diffraction at pH 7. The absorption maximum of D85N at pH 7 is located at 605 nm, which is similar to the acid-blue form of wild-type bR. D85N shows a monophasic CD band, the maximum of which is at 575 nm, although the crystalline arrangement and the trimeric structure is maintained. The acid-blue form of wild-type bR shows a biphasic CD despite the similarity in absorption spectra.
【 授权许可】
Unknown
【 预 览 】
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