Identification of a new b‐type cytochrome from the whitefish Coregonidae eggs

FEBS Letters

Bykhovsky, Vladimir Ya.¹  Artzatbanov, Vladislav Yu.¹  Arutjunjan, Alexander M.²  Valyushok, Dmitry S.¹ 

[1]A.N. Bach Institute of Biochemistry, Leninsky pr. 33, 117071 Moscow, Russian Federation

[2]A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899 Moscow, Russian Federation

关键词: Cytochrome b 560;    Yolk of egg;    Whitefish;   

DOI  :  10.1016/0014-5793(93)80484-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new b-type cytochrome, termed cytochrome b ₅₆₀ according to the wavelength maximum of its α-band in the reduced minus oxidized difference spectrum, was isolated from eggs of whitefish and partially purified by fractionation of the water-soluble moiety of yolk. The absolute absorption spectrum of reduced cytochrome b ₆₅₀ has maxima at 426, 529 and 560 nm and that of the oxidized form at 416 nm. The reduced minus oxidized difference spectrum has maxima at 428, 529 and 560 nm. The midpoint potential of this cytochrome is +193 mV. Based on the MCD spectra of reduced cytochrome and the optical absorption spectra in the visible region of the oxidized cytochrome, it is suggested that the heme iron in cytochrome b ₅₆₀ has two histidine imidazoles as the 5th and 6th axial ligands.

【 授权许可】

Unknown