The role of protein kinase C in the induction of VCAM‐1 expression on human umbilical vein endothelial cells

FEBS Letters

Montgomery, Kevin F.¹  Deisher, Theresa A.²  Haddix, Terri L.¹  Pohlman, Timothy H.¹  Harlan, John M.²  Kaushansky, Kenneth² 

[1] Department of Surgery, University of Washington, Seattle, WA 98195, USA;Department of Medicine/Division of Hematology, Rm. 10, University of Washington Health Sciences, 1959 N.E. Pacific St., Seattle, WA 98195, USA

关键词: Protein kinase C;    Vascular cell adhesion molecule-1;    Tumor necrosis factor-α;    Interleukin-1β;    Lipopolysaccharide;    Human umbilical vein endothelial cell;   

DOI  :  10.1016/0014-5793(93)80354-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of protein kinase C (PKC) in interleukin-1β- (II- 1β)-, tumor necrosis factor-α- (TNF-α)-, and lipopolysaccharide-(LPS)-induced vascular cell adhesion molecule-1 (VCAM-1) expression on human umbilical vein endothelial cells (HUVEC) was studied. PKC inhibition or downregulation diminished VCAM-1 mRNA accumulation and protein expression. Interleukin-1β, TNF-α, and LPS induce nuclear factor (NF)-kB-like binding activity, which precedes VCAM-1 transcription. PKC inhibition did not prevent NF-xB-like binding activity, indicating that this is PKC-independent, and NF-kB-like binding activity is insufficient for transcription of VCAM-1.

【 授权许可】

Unknown