GTP/ggS‐Induced phosphorylation of myosin light chain kinase in smooth muscle

FEBS Letters

Kubota, Yasutaka²  Kamm, Kristine E.¹  Stull, James T.¹  Tang, Da-Chun¹ 

[1] Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, TX 75235-9040, USA;Faculty of Dentistry, Second Department of Oral Surgery, Kyushu University, Fukuoka, 812, Japan

关键词: Smooth muscle contraction;    Myosin light chain;    Myosin light chain kinase;    GTPγS;    Protein phosphatase;    [Ca2*]i;    free intracellular Ca2+ concentration;    KN-62;    1-[N-O-Bis(5-isoquinolinesulfonyl)-N+-methyl-l-tyrosyl]-4-phenylpiperazine;    G-protein;    guanine nucleotide-binding protein;    PSS;    physiological salt solution;    PAGE;    polyacrylamide gel electrophoresis;    PMSF;    phenylmethylsulfonyl fluoride;    EDTA;    ethylenediamine-tetraacetic acid;    MOPS;    3-[N-morpholino]propane-sulfonic acid;    EGTA;    ethyleneglycol-bis (β-aminoethylether)N;    N;    N';    N';    -tetraacetic acid;    k cam;    concentration of Ca2+/calmodulin required for half-maximal activation;    PIPES;    piperazine-N;    N-bis[2-ethane-sulfonic acid];   

DOI  :  10.1016/0014-5793(93)80351-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylation of myosin light chain kinase by a Ca²⁺-dependent protein kinase increases the concentration of Ca²⁺/calmodulin required for half-maximal activation. The Ca²⁺ concentrations required for myosin light chain kinase phosphorylation in permeable smooth muscle are similar to those required for myosin light chain phosphorylation. Both GTPγS and carbachol increase the Ca²⁺ sensitivity of myosin light chain kinase phosphorylation as well as light chain phosphorylation. It is proposed that a similar G-protein mediated mechanism regulates the Ca²⁺-dependent phosphorylation of these two contractile proteins in smooth muscle.

【 授权许可】

Unknown