Prolonged, repetitive calcium transients in rat oocytes fertilized in vitro and in vivo

FEBS Letters

Ben-Yosef, Dalit¹  Shalgi, Ruth¹  Oron, Yoram² 

[1] Department of Embryology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel;Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel

关键词: Calcium transient;    Fertilization;    in vivo;    Fertilization;    in vitro;    Oocyte;    Rat;   

DOI  :  10.1016/0014-5793(93)80344-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Zona-free rat oocytes inseminated with capacitated sperm, under conditions that allow polyspermic fertilization, exhibited a rapid, transient elevation of cellular calcium (from 147 ± 10 to 607 ± 55 nM, n = 19, measured by Fura 2 fluorescence ratio imaging) immediately after sperm attachment. This peak was followed by a series of dramatic calcium transients of high amplitude (maximal 847 ± 32 nM) and frequency (range 2.1 ± 0.07–3.9 ± 0.07 min), which continued for several hours. A similar pattern was seen also in zona-free oocytes fertilized with low sperm density (i.e. producing mainly monospermic attachment) and in zona-enclosed oocytes fertilized in vitro. Moreover, single or repetitive calcium transients were observed in rat oocytes fertilized in vivo. These findings indicate that in normal fertilization in vivo, sperm-oocyte interaction initiates a prolonged train of cyclical calcium changes in the oocyte. This activity may be necessary for the early events in the fertilization process.

【 授权许可】

Unknown