Bovine inositol monophosphatase

FEBS Letters

Gore, M.G.¹  Greasley, P.J.¹ 

[1] Department of Biochemistry, University of Southampton, Bassett Crescent East, Southampton, S09 3TU, UK

关键词: Inositol monophosphatase;    Magnesium;    Lithium;    Phosphate;    Rapid equilibrium dialysis;   

DOI  :  10.1016/0014-5793(93)80308-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rapid equilibrium dialysis has been used to show that recombinant bovine brain inositol monophosphatase binds one equivalent of P_i per subunit of enzyme. P_i is only bound in the presence of Mg²⁺ ions. The dissociation constant for the equilibrium is approximately 50 μM. This value of K _d is independent of the concentration of the Mg²⁺ ions and of the presence or absence of Li⁺ ions. Lithium ions which inhibit the enzyme uncompetitively are not able to support the binding of the P_i to the enzyme. The observation that P_i only binds in the presence of Mg²⁺ ions supports similar conclusions made in experiments which studied the protection of the enzyme from proteolytic degradation and chemical modification.

【 授权许可】

Unknown