Isoprenylation of Rab proteins possessing a C‐terminal CaaX motif

FEBS Letters

Tavitian, Armand¹  Joberty, Gérard¹  Zahraoui, Ahmed¹ 

[1] INSERM U248, Faculté de médecine Lariboisière Saint Louis, 10 Avenue de Verdun, 75010 Paris, France

关键词: Rab small GTPase;    Isoprenylation;    C-terminal CaaX motif;   

DOI  :  10.1016/0014-5793(93)80897-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rab proteins are small GTPases highly related to the yeast Ypti and Sec4 proteins involved in secretion. The Rab proteins were found associated with membranes of different compartments along the secretory and endocytic pathways. They share distinct C-terminal cysteine motifs required for membrane association. Unlike the other Rab proteins, Rab8, Rab11 and Rab13 terminate with a C-terminal CaaX motif similar to those of Ras/Rho proteins. This report demonstrates that Rab8 and Rab13 proteins are isoprenylated in vivo and geranylgeranylated in vitro. Rab11 associates in vitro geranylgeranylpyrophosphate and farnesylpyrophosphate. Our study shows that the CaaX motif is required for isoprenylation.

【 授权许可】

Unknown