Immunohistochemical analysis of rat S‐adenosylmethionine synthetase isozymes in developmental liver

FEBS Letters

Ota, Kazuo²  Horikawa, Saburo¹  Ozasa, Hisashi²  Tsukada, Kinji¹ 

[1] Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Kanda-surugadai, Chiyoda-ku, Tokyo 101, Japan;Kidney Center, Tokyo Women's Medical College, Kawada-cho, Shinjuku-ku, Tokyo 162, Japan

关键词: S-Adenosylmethionine synthelase;    Isozyme;    Immunohistochemical analysis;   

DOI  :  10.1016/0014-5793(93)80894-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mammalian S-adenosylmethionine (AdoMet) synthetase exists as two isozymes, liver-type and kidney(non-hepatic)-type enzymes. The developmental expression of these two isozyme proteins has been investigated in rat liver using immunohistochemical techniques. The liver-type AdoMet synthetase is expressed only in adult liver, but not in fetal liver. On the other hand, the kidney-type AdoMet synthetase is predominantly expressed in fetal liver and faintly detected in adult liver. It was also found that both isozymes were localized to the hepatocytes of rat liver. These results clearly show that AdoMet synthetase isozymes are developmentally regulated within hepatocytes. In addition, in rat kidney we have shown that the kidney-type AdoMet synthetase is predominantly localized to the distal tubule.

【 授权许可】

Unknown