1H assignment and secondary structure determination of human melanoma growth stimulating activity (MGSA) by NMR spectroscopy

FEBS Letters

Reilly, Dorothea³  Fairbrother, Wayne J.¹  Colby, Timothy²  Horuk, Richard² 

[1] Department of Protein Engineering, Genentech Inc., 460 Pt. San Bruno Blvd., South San Francisco, CA 94080-4990, USA;Department of Protein Chemistry, Genentech Inc., 460 Pt. San Bruno Blvd., South San Francisco, CA 94080-4990, USA;Department of Cell Culture, Genentech Inc., 460 Pt. San Bruno Blvd., South San Francisco, CA 94080-4990, USA

关键词: Chemokines;    Melanoma growth stimulating activity (MGSA);    Protein structure;    Nuclear magnetic resonance;   

DOI  :  10.1016/0014-5793(93)80893-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The solution structure of melanoma growth stimulating activity (MGSA) has been investigated using proton NMR spectroscopy. Sequential resonance assignments have been carried out, and elements of secondary structure have been identified on the basis of NOE, coupling constant, chemical shift, and amide proton exchange data. Long-range NOEs have established that MGSA is a dimer in solution. The secondary structure and dimer interface of MGSA appear to be similar to those found previously for the homologous chemokine interleukin-8 [Clore et al. (1990) Biochemistry 29, 1689-1696]. The MGSA monomer contains a three stranded anti-parallel β-sheet arranged in a ‘Greek-key’ conformation, and a C-terininal α-helix (residues 58 69).

【 授权许可】

Unknown