Expression of low‐affinity NGF receptor and trkB mRNA in human SH‐SY5Y neuroblastoma cells

FEBS Letters

Ganter, Ursula²  Schmutz, Béatrice¹  Ehrhard, Patricia B.¹  Bauer, Joachim²  Otten, Uwe¹ 

[1] Department of Physiology, University of Basel, Vesalianum, Vesalgasse 1, CH-4051 Basel, Switzerland;Psychiatrische Universitätsklinik, Hauptstrasse 5, D-7800 Freiburg, Germany

关键词: Brain-derived neurotrophic factor;    Polymerase chain reaction;    Gene expression;    Differentiation;    BDNF;    brain-derived neurotrophic factor;    dbcAMP;    dibutyryl cyclic AMP;    FCS. fetal calf serum;    LNGFR;    low-affinity NGF receptor;    NGF;    nerve growth factor;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/0014-5793(93)80890-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have used the human neuroblastoma cell line SH-SY5Y as a model system to investigate the expression and regulation of the receptors for brain-derived neurotrophic factor (BDNF), a member of the nerve growth factor (NGF) family of neurotrophins. We demonstrate that SH-SY5Y cells express transcripts encoding the low-affinity NGF receptor (LNGFR) and trkB, the signal transducing receptor unit for BDNF. Interaction of BDNF with SH-SY5Y cells increased the transcription of the c-fos gene, showing that these molecules encode functional BDNF receptors. Our findings that differentiating agents such as retinoids and cAMP analogs increased the expression of LNGFR, but decreased trkB mRNA levels, suggest that LNGFR and trkB have different roles during neuronal differentiation.

【 授权许可】

Unknown