The conformation of tRNA genes

FEBS Letters

Goddard, J.P.¹  Carr, T.¹  McLaren, A.¹  Gonos, E.S.¹ 

[1] Department of Biochemistry, University of Glasgow, Glasgow, G12 8QQ, Scotland, UK

关键词: tRNA gene;    Transfer RNA;    Conformation;    Bisulphite;    Transcription;    Cruciform;   

DOI  :  10.1016/0014-5793(93)80268-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been suggested that eukaryotic tRNA genes might adopt a higher order stem and loop structure to facilitate transcription by interaction of their variably spaced intragenic promoter blocks. Using sodium bisulphite, which reacts specifically with cytosine residues in single-stranded nucleic acids, no deamination of C in the TTCGAA sequence of the 3' ICR of a tRNA^Leu gene could be detected under conditions which caused 60% deamination of cytosine residues within the loop region of a synthetic cruciform cloned in the same negatively supercoiled plasmid vector. We conclude that, under these conditions, such structures occur in tRNA genes very rarely, if at all.

【 授权许可】

Unknown