FEBS Letters | |
The conformation of tRNA genes | |
Goddard, J.P.1  Carr, T.1  McLaren, A.1  Gonos, E.S.1  | |
[1] Department of Biochemistry, University of Glasgow, Glasgow, G12 8QQ, Scotland, UK | |
关键词: tRNA gene; Transfer RNA; Conformation; Bisulphite; Transcription; Cruciform; | |
DOI : 10.1016/0014-5793(93)80268-Y | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
It has been suggested that eukaryotic tRNA genes might adopt a higher order stem and loop structure to facilitate transcription by interaction of their variably spaced intragenic promoter blocks. Using sodium bisulphite, which reacts specifically with cytosine residues in single-stranded nucleic acids, no deamination of C in the TTCGAA sequence of the 3' ICR of a tRNALeu gene could be detected under conditions which caused 60% deamination of cytosine residues within the loop region of a synthetic cruciform cloned in the same negatively supercoiled plasmid vector. We conclude that, under these conditions, such structures occur in tRNA genes very rarely, if at all.
【 授权许可】
Unknown
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