Production of recombinant antibodies in lymphoid and non‐lymphoid cells

FEBS Letters

Polanovsky, Oleg L.¹  Deyev, Sergey M.¹  Lieber, Andre²  Radko, Boris V.¹ 

[1] Laboratory of Molecular Genetic Immunology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117984 Moscow, Russian Federation;Max-Delbruck-Centrum, Robert-Rossie-Strasse 10, O-1115 Berlin-Buch, Germany

关键词: Antibody engineering;    Gene tandem expression;    Phage T7 promoter;    Sp2/0 cell;    CHO cell;   

DOI  :  10.1016/0014-5793(93)80253-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A recombinant tandem of ‘chimeric’ mouse/human immunoglobulin (Ig) genes was constructed and inserted into plasmid pGEM 1 under the control of the T7 bacteriophage RNA polymerase promoter. Lymphoid (Sp2/0) and non-lymphoid (CHO) cell lines used for transfection contained in their genomes a semisynthetic gene of T7 RNA polymerase and steadily expressed this enzyme. It was shown for the first time that a stable polycystronic transcription of the Ig gene tandem occurs under the control of a single T7 phage promoter, both in lymphoid and non-lymphoid cells. Synthesis of κ-light and ε-heavy Ig chains and functionally active antibodies was observed in the above-mentioned transfected cell lines.

【 授权许可】

Unknown