Observation of inter‐subunit nuclear Overhauser effects in a dimeric protein

FEBS Letters

Breg, J.N.¹  Kaptein, R.¹  Burgering, M.J.M.¹  Caffrey, M.¹  Boelens, R.¹ 

[1] Bijvoet Center for Biomolecular Research, Padualaan 8, 3584 CH Utrecht, The Netherlands

关键词: NMR;    Arc repressor;    Dimer;    Inter-subunit NOE;    2D;    two-dimensional;    MD;    molecular dynamics;    NMR;    nuclear magnetic resonance;    NOE;    nuclear Overhauser effect;    NOESY;    2D NOE spectroscopy;    TPPI;    time proportional phase increment;   

DOI  :  10.1016/0014-5793(93)80929-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform ¹⁵N and ¹³C isotope labelling. This is accomplished by doubly filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc represser and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.

【 授权许可】

Unknown