期刊论文详细信息
FEBS Letters | |
Observation of inter‐subunit nuclear Overhauser effects in a dimeric protein | |
Breg, J.N.1  Kaptein, R.1  Burgering, M.J.M.1  Caffrey, M.1  Boelens, R.1  | |
[1] Bijvoet Center for Biomolecular Research, Padualaan 8, 3584 CH Utrecht, The Netherlands | |
关键词: NMR; Arc repressor; Dimer; Inter-subunit NOE; 2D; two-dimensional; MD; molecular dynamics; NMR; nuclear magnetic resonance; NOE; nuclear Overhauser effect; NOESY; 2D NOE spectroscopy; TPPI; time proportional phase increment; | |
DOI : 10.1016/0014-5793(93)80929-O | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform 15N and 13C isotope labelling. This is accomplished by doubly filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc represser and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.
【 授权许可】
Unknown
【 预 览 】
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