Uridylylation of the PII protein in Rhizobium leguminosarum

FEBS Letters

Colonna-Romano, Sergio¹  Manco, Giuseppe¹  Lamberti, Alessandro¹  laccarino, Maurizio¹  Defez, Roberto¹  Amar, Mohamed¹  Patriarca, Eduardo Jorge¹  Bernard, Paola¹ 

[1] International Institute of Genetics and Biophysics, CNR Via Marconi 10, 80125 Naples, Italy

关键词: Uridylylation;    GS cascade;    PII protein;    CETAB;    cetyl trimethyl ammonium bromide;    SVPD;    snake venom phosphodiesterase;   

DOI  :  10.1016/0014-5793(93)80927-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Permeabilization with cetyl trimethyl ammonium bromide was used to study the post-translational modification of the P_II protein in Rhizobium leguminosarum. Upon incubation with radioactive UTP a single band was obtained after SDS-PAGE and autoradiography. RNase resistance and snake venom phosphodiesterase sensivity showed that radioactivity was bound through a phosphodiester bond to a protein which was absorbed by an antiserum specific for the P_II protein. Uridylylation of the P_II protein was shown to be dependent on the modifications of the glutamine/α-ketoglutarate ratio.

【 授权许可】

Unknown