Cooperative homodimeric hemoglobin from Scapharca inaequivalvis

FEBS Letters

Piro, Maria Cristina¹  Gambacurta, Alessandra¹  Ascoli, Franca¹ 

[1] Department of Experimental Medicine and Biochemical Sciences, Tor Vergata University, via O. Raimondo, 00173 Rome, Italy

关键词: Globin cDNA;    Reverse transcriptase-PCR;    Expression in E. coli;    Recombinant hemoglobin;    Scapharca inaequiralvis;   

DOI  :  10.1016/0014-5793(93)80926-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The overexpression of the fully functional, cooperative homodimeric hemoglobin of the bivalve mollusc. Scapharca inaequivalvis, has been accomplished in E. coli from its cDNA. The latter was isolated by PCR amplification of total RNA and sequenced. The cDNA-derived sequence differed by a single amino acid when compared to that previously obtained from purified protein. Interest in this hemoglobin resides in the unique assemblage of the two identical subunits, with the heme groups facing each other in the inside of the molecule, opposite to that occurring in vertebrate hemoglobins. The results presented here are the basis for future studies of structure/function relationships by site directed mutagenesis.

【 授权许可】

Unknown